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. 2019 Dec 6;10:2889. doi: 10.3389/fimmu.2019.02889

Figure 8.

Figure 8

The STING pathway was involved in the enhanced HIV infection of HSV-2 exposed DCs. IFI16/cGAS inhibitory oligonucleotide A151 was delivered intracellularly by DOTAP to DCs. Cells were then exposed to free HSV-2 (HSV) or complement opsonized virus (CHSV) for 2 h before infection with HIV or complement opsonized HIV (CHIV) for (A) 6 h (B) or 22 h. (B) STING protein inhibitor HPV 18 E7 proteins was delivered intracellularly by DOTAP to DCs. Cells were then exposed to free HSV or CHSV for 2 h before infection with HIV or CHIV for 22 h. HIV gag transcript and IFN-β expression were evaluated by qPCR. (C) THP1 cells with STING knockout or wildtype were exposed to free HSV-2 or complement opsonized virus (CHSV) for 2 h then infected with HIV or complement opsonized HIV (CHIV) for 22 h. HIV gag transcript and IFN-β expression were evaluated by qPCR. HIV gag data were normalized to free virus set as 1 and IFN-β data were normalized to mock set as 1. *p < 0.05; **p < 0.005; ***p < 0.0005. N = 5–8.