Table 2.

Validated Candidate housekeeping genes and primers for M. incognita.

Gene symbol	Forward	Reverse	M. hapla gene ID	M. incognita homologue loci	Annotation
Actin1	GGGTATGGAATCTGCTGGTATT	CATGGTCGTACCTCCAGAAAG	MhA1_Contig4.frz3.gene2	Minc3s00730g16611 Minc3s00960g19311	Actin
Disu	CACCGCTAATGAAGTTGAAGATG	CGCTCTCCAGTGTAGTCAATAA	MhA1_Contig165.frz3.gene9	Minc3s01268g22290 Minc3s02350g29607 Minc3s04154g35616	protein disulfide-isomerase
Minc04440	GCCGCCATCTTGGCTATTA	GGTGGGACCAAATCCTTGTAT	MhA1_Contig113.frz3.gene11	Minc3s00096g04440 Minc3s00113g04971 Minc3s00259g08816	not annotated
Poly	ACGTCCAGAGAATCTGACAAAC	CCACGGCAATAGCGAGATAC	MhA1_Contig123.frz3.gene87	Minc3s01682g25559 Minc3s03281g33396	Polyadenylate-binding protein
ELF	CTCCTGGACACGTTGACTTC	GCACACAAACTCCTGAAACAC	MhA1_Contig256.frz3.fgene4	Minc3s00468g12850 Minc3s00594g14836 Minc3s01848g26725 Minc3s02628g30935	Elongation factor 2
PTP	GGAAAGCATTTTCACCTGCGA	ACGGGTTGCCAACAGATGAA	MhA1_Contig342.frz3.gene42	Minc3s00073g03598 Minc3s00981g19546 Minc3s03830g34926	protein transport
Skinase	AGCGTTTGGTTGCTCTCTCA	GACTCTCCCACGCAAGGAAT	MhA1_Contig647.frz3.gene12	Minc3s00176g06827 Minc3s00279g09289 Minc3s03755g34706 Minc3s09595g43359 Minc3s11722g45107	Serine/threonine-protein kinase smg-1