Table 1.
Analytes | DxC 700 AU Methodology | Siemens Dimension Vista 500 Methodology |
---|---|---|
Critical Care | ||
Na | Indirect ISE | Indirect ISE |
K | Indirect ISE | Indirect ISE |
Cl | Indirect ISE | Indirect ISE |
CO2 | PEP-C Enzymatic | PEP-C Enzymatic |
Albumina | BCG | BCP |
BUN/Urea nitrogen | Urease GLDH Enzymatic | Urease GLDH Enzymatic |
Calciuma | Arsenazo III, Bichromatic | Cresolphthalein Complexone, Bichromatic |
Creatininea | Kinetic Modified Jaffe, IDMS Traceable | Kinetic Creatininase/Peroxide, IDMS Traceable |
Glucose | Hexokinase-UV/NAD-NADH | Hexokinase-UV/NAD-NADH |
Phosphorus | Phosphomolybdate-UV | Phosphomolybdate-UV |
Total Protein | Biuret | Biuret |
General Chemistry | ||
ALP | p-NPP/AMP IFCC | p-NPP/AMP IFCC |
ALT | L-Alanine-KG/NADH to NAD IFCC | L-Alanine-KG/NADH to NAD IFCC |
Ammonia | Glutamate Dehydrogenase | Glutamate Dehydrogenase |
Amylase | CNPG3 Enzymatic | CNPG3 Enzymatic IFCC |
AST | L-Aspartate KG/NADH to NAD, UV | L-Aspartate KG/NADH to NAD, UV |
Cholesterol | Cholesterol Esterase/Peroxidase, Bichromatic | Cholesterol Esterase/Cholesterol Oxidase, Polychromatic |
CK | CP/ADP/NADP to NADPH IFCC | CP/ADP/NADP to NADPH IFCC |
DBILa | Diazotized DPD | Diazotized Sulfanilic Acid |
GGT | GCNA IFCC | GCNA IFCC |
HDL-Chol | Accelerator Selective Detergent, Bichromatic | Liquid Selective Detergent, Bichromatic |
Irona | TPTZ Colorimetric | Ferene without Prior Protein Removal |
Lactatea | Lactate to Pyruvate/H2O2/Peroxidase | Lactate to Pyruvate/NAD to NADH |
LD | L-Lactate/NAD to NADH IFCC | L-Lactate/NAD to NADH IFCC |
LDL-Chol | Cholesterol Esterase/Peroxidase, Bichromatic | Cholesterol Esterase/Peroxidase, Bichromatic |
Lipasea | Imamura Di-Glyceride | Methyl Resorufin Ester Substrate |
Magnesium | Colorimetric Xylidyl Blue | Colorimetric Methylthymol Blue |
TBILa | Diazotized DPD | Diazotized Sulfanilic Acid |
Triglyceride | Enzymatic GPO-Trinder | Enzymatic GPO-Trinder |
Uric Acid | Uricase | Uricase |
Proteins | ||
Complement C3a | Immunoturbidimetric | Nephelometric |
Complement C4a | Immunoturbidimetric | Nephelometric |
CRPa | Immunoturbidimetric | Nephelometric |
CRP-hsa | Latex Particle Immunoturbidimetric | Nephelometric |
Ferritina | Latex Particle Immunoturbidimetric | Homogeneous, Sandwich Chemiluminescent Immunoassay |
IgAa | Immunoturbidimetric | Nephelometric |
IgGa | Immunoturbidimetric | Nephelometric |
IgMa | Immunoturbidimetric | Nephelometric |
Prealbumina | Immunoturbidimetric | Nephelometric |
RFa | Immunoturbidimetric | Nephelometric |
Transferrina | Immunoturbidimetric | Nephelometric |
Urines | ||
U-Albumina | Immunoturbidimetric | Nephelometric |
U-BUN | Urease GLDH Enzymatic | Urease with GLDH |
U-Calciuma | Arsenazo III, Bichromatic | Cresolphthalein Complexone, Bichromatic |
U-Chloride | Indirect ISE | Indirect ISE |
U-Creatininea | Kinetic Modified Jaffe, IDMS Traceable | Kinetic Creatininase/Peroxide, IDMS Traceable |
U-Glucose | Hexokinase-UV/NAD | Hexokinase-UV/NAD |
U-Phosphorus | Phosphomolybdate-UV | Phosphomolybdate-UV |
U-Potassium | Indirect ISE | Indirect ISE |
U-Sodium | Indirect ISE | Indirect ISE |
U-Total Protein | Pyrogallol Red | Pyrogallol Red |
CSF | ||
CSF-Glucose | Hexokinase-UV/NAD | Hexokinase-UV/NAD |
CSF-Total Protein | Pyrogallol Red | Pyrogallol Red |
This table includes a list of methodologies for each assay tested on each platform. The methodologies were taken from the respective manufacturer’s assay IFU.
Footnoted assays indicate differing methodologies.