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. 2019 Nov 20;18:e00148. doi: 10.1016/j.plabm.2019.e00148

Table 1.

DxC 700 AU to siemens dimension vista 500 methodology comparisons.

Analytes DxC 700 AU Methodology Siemens Dimension Vista 500 Methodology
Critical Care
 Na Indirect ISE Indirect ISE
 K Indirect ISE Indirect ISE
 Cl Indirect ISE Indirect ISE
 CO2 PEP-C Enzymatic PEP-C Enzymatic
 Albumina BCG BCP
 BUN/Urea nitrogen Urease GLDH Enzymatic Urease GLDH Enzymatic
 Calciuma Arsenazo III, Bichromatic Cresolphthalein Complexone, Bichromatic
 Creatininea Kinetic Modified Jaffe, IDMS Traceable Kinetic Creatininase/Peroxide, IDMS Traceable
 Glucose Hexokinase-UV/NAD-NADH Hexokinase-UV/NAD-NADH
 Phosphorus Phosphomolybdate-UV Phosphomolybdate-UV
 Total Protein Biuret Biuret



General Chemistry
 ALP p-NPP/AMP IFCC p-NPP/AMP IFCC
 ALT L-Alanine-KG/NADH to NAD IFCC L-Alanine-KG/NADH to NAD IFCC
 Ammonia Glutamate Dehydrogenase Glutamate Dehydrogenase
 Amylase CNPG3 Enzymatic CNPG3 Enzymatic IFCC
 AST L-Aspartate KG/NADH to NAD, UV L-Aspartate KG/NADH to NAD, UV
 Cholesterol Cholesterol Esterase/Peroxidase, Bichromatic Cholesterol Esterase/Cholesterol Oxidase, Polychromatic
 CK CP/ADP/NADP to NADPH IFCC CP/ADP/NADP to NADPH IFCC
 DBILa Diazotized DPD Diazotized Sulfanilic Acid
 GGT GCNA IFCC GCNA IFCC
 HDL-Chol Accelerator Selective Detergent, Bichromatic Liquid Selective Detergent, Bichromatic
 Irona TPTZ Colorimetric Ferene without Prior Protein Removal
 Lactatea Lactate to Pyruvate/H2O2/Peroxidase Lactate to Pyruvate/NAD to NADH
 LD L-Lactate/NAD to NADH IFCC L-Lactate/NAD to NADH IFCC
 LDL-Chol Cholesterol Esterase/Peroxidase, Bichromatic Cholesterol Esterase/Peroxidase, Bichromatic
 Lipasea Imamura Di-Glyceride Methyl Resorufin Ester Substrate
 Magnesium Colorimetric Xylidyl Blue Colorimetric Methylthymol Blue
 TBILa Diazotized DPD Diazotized Sulfanilic Acid
 Triglyceride Enzymatic GPO-Trinder Enzymatic GPO-Trinder
 Uric Acid Uricase Uricase



Proteins
 Complement C3a Immunoturbidimetric Nephelometric
 Complement C4a Immunoturbidimetric Nephelometric
 CRPa Immunoturbidimetric Nephelometric
 CRP-hsa Latex Particle Immunoturbidimetric Nephelometric
 Ferritina Latex Particle Immunoturbidimetric Homogeneous, Sandwich Chemiluminescent Immunoassay
 IgAa Immunoturbidimetric Nephelometric
 IgGa Immunoturbidimetric Nephelometric
 IgMa Immunoturbidimetric Nephelometric
 Prealbumina Immunoturbidimetric Nephelometric
 RFa Immunoturbidimetric Nephelometric
 Transferrina Immunoturbidimetric Nephelometric



Urines
 U-Albumina Immunoturbidimetric Nephelometric
 U-BUN Urease GLDH Enzymatic Urease with GLDH
 U-Calciuma Arsenazo III, Bichromatic Cresolphthalein Complexone, Bichromatic
 U-Chloride Indirect ISE Indirect ISE
 U-Creatininea Kinetic Modified Jaffe, IDMS Traceable Kinetic Creatininase/Peroxide, IDMS Traceable
 U-Glucose Hexokinase-UV/NAD Hexokinase-UV/NAD
 U-Phosphorus Phosphomolybdate-UV Phosphomolybdate-UV
 U-Potassium Indirect ISE Indirect ISE
 U-Sodium Indirect ISE Indirect ISE
 U-Total Protein Pyrogallol Red Pyrogallol Red



CSF
 CSF-Glucose Hexokinase-UV/NAD Hexokinase-UV/NAD
 CSF-Total Protein Pyrogallol Red Pyrogallol Red

This table includes a list of methodologies for each assay tested on each platform. The methodologies were taken from the respective manufacturer’s assay IFU.

a

Footnoted assays indicate differing methodologies.