Table 1.
The experimental design for optimizing the extraction process using choline chloride-glycerol-based natural deep eutectic solvent (NADES) to extract bioactive substances from cinnamon and sappan wood.
| Extraction parameters | Units | Symbol | Range and level |
|||||
|---|---|---|---|---|---|---|---|---|
| Low |
Medium |
High |
||||||
| A | B | A | B | A | B | |||
| Ratio of glycerol vs ChCl in NADES (HBD-HBA molarity ratio) | %HBD | x1 | 66.00 | 66.00 | 50.00 | 33.00 | 33.00 | 20.00 |
| Ratio of NADES vs sample powders (liquid-solid ratio) | %Liquid | x2 | 66.00 | 83.30 | 75.00 | 90.90 | 80.00 | 93.75 |
| Presence of water in NADES | %Water content | x3 | 10.00 | 20.00 | 30.00 | 50.00 | 50.00 | 80.00 |
Bioactive contents are expressed as the mean ± SEM of two replicates. The extraction conditions that produced the (a) highest and (b) lowest bioactive compound yields are labeled.