. 2019 Dec 9;5(12):e02915. doi: 10.1016/j.heliyon.2019.e02915

Table 2.

The results of the determination bioactive substances contained in Cinnamomum burmanii NADES extracts from experimental design suggested by RSM.

Run	Independent Variables					Responses
	(x₁) Ratio of Glycerol vs ChCl in NADES (%)		(x₂) Ratio of NADES vs Sample powders (%)		(x₃) Percentage of water added (%)	R1: Trans-cinnamaldehyde levels (μg/ml)		R2: Coumarin levels (μg/ml)
	Glycerol	ChCl	NADES	Powder	(x₃) Percentage of water added (%)	Predicted	Actual	Predicted	Actual
1	33.33	66.67	93.75	6.25	50.00	830.11	858.72 ± 11.33	678.37	643.35 ± 13.02
2	56.66	43.34	93.75	6.25	80.00	416.83	453.59 ± 1.17^b	617.62	616.76 ± 7.42^b
3	80.00	20.00	83.33	16.67	50.00	1710.55	1681.94 ± 12.18	1694.05	1729.07 ± 18.09
4	80.00	20.00	88.54	11.46	80.00	906.97	956.60 ± 9.37	850.63	930.09 ± 11.18
5	33.33	66.67	88.54	11.46	20.00	1175.83	1126.20 ± 24.56	952.15	872.69 ± 23.75
6	33.33	66.67	88.54	11.46	80.00	1240.13	1174.75 ± 21.24	1107.12	1143.00 ± 27.19
7	56.66	43.34	83.33	16.67	50.00	1041.86	1035.24 ± 29.71	1152.79	1173.40 ± 39.08
8	33.33	66.67	83.33	16.67	50.00	1820.93	1907.32 ± 96.59^a	1702.26	1780.87 ± 112.51^a
9	56.66	43.34	83.33	16.67	20.00	1566.13	1529.36 ± 6.06	1734.82	1735.68 ± 5.40
10	80.00	20.00	93.75	6.25	50.00	686.054	599.66 ± 0.78	720.25	641.64 ± 2.20
11	56.66	43.34	88.54	11.46	50.00	1041.86	972.21 ± 2.48	1152.79	1106.11 ± 3.76
12	56.66	43.34	88.54	11.46	50.00	1041.86	1118.14 ± 20.52	1152.79	1178.87 ± 20.45
13	56.66	43.34	83.33	16.67	80.00	1418.43	1397.41 ± 5.74	1408.93	1294.45 ± 5.74
14	56.66	43.34	93.75	6.25	20.00	552.41	573.43 ± 5.23	528.45	642.93 ± 6.34
15	80.00	20.00	88.54	16.67	20.00	1254.55	1319.93 ± 5.35	1242.32	1206.44 ± 4.30

Bioactive contents are expressed as mean ± SEM (Standard Error Measurement) of two replicates. The results are labeled shows extraction conditions that produce the (^a) highest and (^b) lowest bioactive compounds.