Table 1.
List of R. sphaeroides strains and their CoQ10 production in shake-flask fermentationa.
| Strain | Descriptions | CoQ10 production (mg/L) | Plasmid source/Ref. |
|---|---|---|---|
| HY01 | CoQ10 industrial strain R. sphaeroides | 192.2 ± 3.7 | |
| HY01-pBBR | HY01 containing pBBR1MCS2 (plasmid control) | 192.9 ± 5.9 | [26] |
| MEP pathway overexpression | |||
| HY01-idi | idi overexpression in HY01 | 111.2 ± 4.4 | This study |
| HY01-dxs | dxs overexpression in HY01 | 159.5 ± 5.1 | This study |
| Quinone modification pathway overexpression | |||
| HY01-ubiCA | ubiC, ubiA overexpression in HY01 | 105.5 ± 4.9 | This study |
| HY01-ubiF | ubiF overexpression in HY01 | 86.5 ± 0.7 | This study |
| HY01-ubiH | ubiH overexpression in HY01 | 168.0 ± 8.5 | This study |
| HY01-ubiE | ubiE overexpression in HY01 | 210 ± 3.5 | This study |
| HY01-ubiG | ubiG overexpression in HY01 | 83.5 ± 3.5 | This study |
| MEP and quinone modification pathway optimization | |||
| HY01-MQc | HY01 containing pMCS-MQc (dxs, dxr, idi, ispD, ubiE, ubiG, lacIqRBSc) | 160.8 ± 1.1 | [2] |
| HY01-MQe | HY01 containing pMCS-MQc (dxs, dxr, idi, ispD, ubiE, ubiG, lacIqRBSe) | 90.5 ± 1.2 | [2] |
a
Detail information about construction and fermentation of these genetic engineered strains is provided in supplementary materials. Data are expressed as mean ± standard deviation (SD).