Figure 3.

The Effect on Splicing of Some Genes and Identification of Apigenin- and Luteolin-Targeted Proteins

(A) Schematic of β-globin construct containing the CMV promoter and BGH poly(A) site is presented. Horizontal lines indicate introns, and boxes indicate exons.

(B) The localization of β-globin mRNA was observed. Bulk poly(A)⁺ RNA (green), β-globin mRNA (red), and chromosomal DNA (blue) were visualized in U2OS cells. Scale bar, 10 μm.

(C) The splicing pattern of β-globin gene was observed by RT-PCR using total RNA. The digit panel below the photo shows the percentage of unspliced mRNA band intensity and representative results of triplicate experiments. DNA size in base pairs (b.p.) is indicated on the left side. Control: marker for unspliced β-globin mRNA. RT: reverse transcription.

(D) The change of splicing pattern of Caspase-9 and c-FLIP. RT-PCR was performed using total RNA. DNA size in base pairs (b.p.) is indicated on the right side. The digit panels below the photo show the percentage of the indicated isoform and representative results of triplicate experiments.

(E) Gene ontology analysis of proteins bound with apigenin or luteolin is shown. Proteins with protein score >0 in Tables S1 and S2 were uploaded to the DAVID database for GO analysis.

See also Figures S2–S4.