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. 2019 Nov 20;22:336–352. doi: 10.1016/j.isci.2019.11.033

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© 2019 The Authors

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

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Localization of mRNAs with Retained Intron Induced by Apigenin and Luteolin

(A) Schematic of mini gene constructs with β-globin FISH probe tag. The CMV promoter and BGH poly(A) sites are shown. DNA size in base pairs (b.p.) is indicated on the left side. The digit panels below the photo show the percentage of unspliced mRNA band intensity and representative results of triplicate experiments.

(B) The localization of target mRNA was observed. Bulk poly(A)⁺ RNA (green), target mRNA (red), and chromosomal DNA (blue) were visualized in U2OS cells. Scale bar, 10 μm.