Figure 8.

Inhibition of mRNA Processing and Proliferation of Tumorigenic Cells by Apigenin and Luteolin

(A) Bulk poly(A)⁺ RNA (red) and chromosomal DNA (blue) were visualized in HeLa, MCF7, and HaCaT cells. Cells were treated with the indicated concentrations of each compound for 24 h. Scale bar, 50 μm. In lower panels, the signal intensities of bulk poly(A)⁺ RNA in the whole cell and in the nucleus were quantified using ImageJ (n = 35). Boxes show median (center line) and upper and lower quartiles. Whiskers show the lowest and highest values. Statistical analysis was performed using one-way ANOVA followed by Dunnett's test. ***p < 0.001.

(B) Proliferation of U2OS and HaCaT cells treated with the indicated concentrations of each compound for 24, 48, or 72 h. Cell viability was determined by MTT assay. Cell viability in comparison with that of DMSO-treated cells, which was set to 100%, is reported as mean ± SD of six independent experiments.

See also Figure S9.