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. 2019 Nov 28;29:101392. doi: 10.1016/j.redox.2019.101392

Fig. 3.

Fig. 3

Caspase-8 mediated the apoptosis induced by oroxylin A in SMMC-7721 cells. (A) The caspase-8 activity was measured in SMMC-7721 cells after 16, 32 and 64 μM oroxylin A treatment. (B) Induction of apoptosis was measured by Annexin-V/PI double-staining assay in SMMC-7721 cells after treatment of 50 μM Z-IETD-FMK and 64 μM oroxylin A. (C) The levels of cleaved caspase-3, caspase-9 and PARP were assessed by Western blot in SMMC-7721 cells after treatment of 50 μM Z-IETD-FMK and 64 μM oroxylin A. (D) The levels of FADD was assessed by Western blot in SMMC-7721 cells after knockdown of FADD. (E) Induction of apoptosis was measured by Annexin-V/PI double-staining assay in SMMC-7721 cells after knockdown of FADD. (F) The levels of cleaved caspase-8, cleaved caspase-3, caspase-9 and PARP were assessed by Western blot in SMMC-7721 cells after knockdown of FADD. Data are presented as mean ± SD. *P < 0.05, **P < 0.01 compared with DMSO group.