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. 2019 Nov 28;29:101392. doi: 10.1016/j.redox.2019.101392

Fig. 4.

Fig. 4

Knockdown of p62 attenuates oroxylin A-induced apoptosis and caspase-8 activation in hepatocellular carcinoma cells. (A) The levels of p62 was assessed by western blot in A549, H460, MCF-7, MDA-MB-231, LN229, U87, HCT116, RKO, SMMC-7721, HepG2 and MHCC-97H cells. (B) The levels of p62 was assessed by western blot in SMMC-7721, HepG2 and MHCC-97H cells after transfection of p62 shRNA. (C) Effect of certain concentration oroxylin A on the surviving fraction were measured by MTT assay in SMMC-7721, HepG2 and MHCC-97H cells after knockdown of p62. (D) MTT assay was used to detect cell surviving fraction after culture for 12 h, 24 h, 48 h and 72 h in SMMC-7721, HepG2 and MHCC-97H cells. (E) Induction of apoptosis was measured by Annexin-V/PI double-staining assay in SMMC-7721, HepG2 and MHCC-97H cells after knockdown of p62. (F) The caspase-8 activity was measured in SMMC-7721 cells after knockdown of p62. (G) The levels of cleaved caspase-8 was assessed by western blot in SMMC-7721 cells after knockdown of p62. Data are presented as mean ± SD. *P < 0.05, **P < 0.01.