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. 2019 Nov 28;29:101392. doi: 10.1016/j.redox.2019.101392

Fig. 6.

Fig. 6

Oroxylin A-driven p62 cleavage down-regulated Nrf2 to induce oxidative stress. (A) Domain composition of human p62/SQSTM1 showing the caspase-8 cleavage site (not to scale). Blue arrows point to regions for interactions with the indicated proteins. (B) The IL-1β and IL-6 mRNA was measured by real-time PCR following 64 μM oroxylin A treatment or p62 knockdown in SMMC-7721 cells. (C) The HO-1, SOD1 and NQO-1 mRNA was measured by real-time PCR following 64 μM oroxylin A treatment or p62 knockdown in SMMC-7721 cells. β-actin was used as internal control. The relative levels were calculated to β-actin mRNA expression. (D) The levels of Nrf2, HO-1 and NQO-1 were assessed by western blot following 64 μM oroxylin A treatment or p62 knockdown in SMMC-7721 cells. (E) The ROS level was assessed following 64 μM oroxylin A and 50 μM Z-IETD-FMK treatment in SMMC-7721 cells. Data are presented as mean ± SD. *P < 0.05, **P < 0.01. (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)