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. 2019 Dec 6;10:2860. doi: 10.3389/fimmu.2019.02860

Figure 1.

Figure 1

miR-1294 effects NFḱB-induced SEAP in TLR-8 expressing cells. (A) HEK-Blue™hTLR-8 cells (Invivogen, San Diego, CA), a stable commercial cell line co-transfected with hTLR-8 gene and an optimized secreted embryonic alkaline phosphatase (SEAP) reporter gene were incubated with miR-1294 mimic for 24 h. (B) HEK-Blue™hTLR-8 cells (Invivogen, San Diego, CA), were incubated with miR-1294 mimic or controls for 18 or 24 h. Negative controls were HEK-Blue™TLR8 cells alone and HEK-Blue™hTLR cells incubated with ssRNA4. Positive controls were HEK-Blue™hTLR8 cells incubated with ssRNA40 were and transfection controls. SEAP levels were quantified by spectrophotometry at 620 nm. Bars represent the mean of the three independent experiments with SEM. All experiments were replicated three times. Data analyzed by one-way ANOVA and Dunnet's post-hoc test. **p < 0.01; ***p < 0.001.