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EMSA-mediated assessment of the binding of NCp7 toward the HIV-1 U3-III+IV RNA G4. (A) Increasing concentrations of NCp7 (7.5, 15, 37.5, 75, 112.5, 150, and 300 nM) were incubated with RNA U3-III+IV G4 folded in 50 mM KCl buffer. C indicates protein/oligonucleotide complex. (B) Quantification of the free G4 band shown in panel (A) (average of two experiments; bars represent standard deviation).
