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CD analysis of NCp7-mediated U3-III+IV RNA G4 unfolding. CD melting and annealing curves of the G4-folded RNA U3-III+IV in the presence/absence of 10-fold excess of NCp7 in 50 mM K+ and CD melting curves of the G4-folded RNA U3-III+IV in the presence of the G4-ligand B19 (8 μM) and in the presence/absence of NCp7 (10×). The molar ellipticity at the peak wavelength (265 nm) is shown as a function of the temperature.
