Figure 5.
NK cells stimulated with caIKKβ-DCs can kill K562 cells.
Cytokine-matured DCs were electroporated either with caIKKβ-RNA or, as a control, were mock electroporated. (a) Transfected dendritic cells (DCs) were co-cultured with fresh autologous peripheral blood mononuclear cells (PBMCs) at a ratio of 1:2 (final concentrations: 1 × 106 DCs/ml and 2 × 106 PBMCs/ml) or 1:10 (final concentrations: 2 × 105 DCs/ml and 2 × 106 PBMCs/ml) and incubated for 1 week. The cytolytic capacity of the resulting cell population was determined in a 51chromium release assay. The K562 cell line was used as target at the indicated effector to target ratios. Average values ± SEM of three independent donors, each analyzed in triplicates, are shown; for original data see Supplemental Table S9. (b) Transfected DCs were co-cultured with fresh autologous NK cells at a ratio of 5:1 (final concentrations: 1 × 106 DCs/ml and 2 × 105 NK cells/ml) and 1:1 (final concentrations: 1 × 106 DCs/ml and 1 × 106 NK cells/ml) and incubated for 1 week. The lytic capacity of the resulting NK cells was determined as depicted in (a). Average values ± SEM of three independent donors, each analyzed in triplicates, are shown; for original data, see Supplemental Table S10. p values were calculated to the respective mock condition using the paired Student’s t test, **p ⩽ 0.01, *p ⩽ 0.05, numbers indicate p values of 0.05 ⩽ p ⩽ 0.1.