Figure 3.

iRhom2 KO mice fed with HFD have reduced adipose tissue depots and smaller adipocytes. A-O Weight (A-E), adipocyte area (F, H, J, L, N), and representative photographs of H-E staining (200x magnification) (G, I, K, M, O) of subcutaneous (sWAT) (A, F, G), mesenteric (B, H, I), epididymal (eWAT) (C, J, K), retroperitoneal (D, L, M) white adipose tissues, and brown adipose tissue (BAT) (E, N, O) from iRhom2 KO and WT mice fed with SD and HFD for 30 weeks (photographs only from HFD-fed mice samples). Three independent HFD-induced obesity experiments, with 11–12 WT and 9–11 KO mice. The analysis of the SD-fed mice was performed on 3 groups of 4–7 mice per genotype. The average WAT adipocyte size [average cross-sectional area per cell (μm²)] was determined using a macro on H-E staining of adipose tissue from 3 HFD-fed animals and 1–3 SD-fed mice per genotype per experiment (3 experiments). The average BAT adipocyte size was calculated as described previously [83] in adipose tissue samples from the SD and HFD-fed animals described above. Scale bar = 100 μm. Error bars represent SEM; * represents p < 0.05, ** represents p < 0.01, *** represents p < 0.001, **** represents p < 0.0001.