Figure 5.

Targeting of eIF4A in BCSCs derived from MDA-Bone-Un cells affects the pluripotency transcription factors, ALDH1A1, drug transporter ABCB1 and induced apoptotic cell death. (A) Representative immunoblot showing the dose-dependent effects of RocA on the levels of eIF4A, molecular targets downstream of eIF4A, pluripotency transcription factors and BCSC markers ALDH1A1 and CD44 (n = 3). (B) The given immunoblot is obtained following RocA treatment of MDA-Bone-Un derived BCSCs at 45 nM for a period of 72 h. The lysates from three biological replicates were pooled and assessed for the expression of SOX2, OCT4, and ABCB1. Fold change in the levels of proteins is indicated above the blots with DMSO control being normalized to 1. The graph following the immunoblot represents the densitometry values normalized to their respective loading controls. The graph corresponding to (A) shows the spread of the data along with its statistical significance. The indicated values are obtained by normalizing the densitometry intensity value with their corresponding loading controls. *p ≤ 0.05, **p ≤ 0.01, ***p ≤ 0.001 ns-not significant. The graph corresponding to (B) shows the trend of SOX2, OCT4, and ABCB1 from 3 biological replicates pooled and analyzed.