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Schematic of domesticating V. stipulacea. After two rounds of EMS treatment, 3,000 M2M1 plants were selected to collect M2M2 seeds (six per line). The 18,000 M2M2 plants were grown in a field, where pod shattering mutants were screened for. Three pods (∼20 seeds) per plant was harvested, which were tested for seed-imbibition screening.
