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. 2019 Dec 12;50:109. doi: 10.1186/s13567-019-0725-0

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© The Author(s) 2019

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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PCR analysis of the lsr mutant strain APEC94Δlsr(Cm) and lsr complementary strain lsr-APEC94Δlsr. A Schematic chart of the strategy for producing the lsr operon deletion mutant. The lsr operon was deleted by replacing the partial gene sequence with a chloramphenicol resistance cassette. The primers used for confirmation of the lsr deletion are also indicated. B Identification of the lsr mutant strain APEC94Δlsr(Cm) and lsr complementary strain lsr-APEC94Δlsr. M1: 5000 DNA marker; M2: 2000 DNA marker; Lane 1: The wild-type strain APEC94 showed no PCR product using primers lsr-UF/lsr-DR because the amplification time was limited beyond the amplification efficiency of DNA polymerase; Lane 2: The mutant strain APEC94Δlsr(Cm) showed a 2414-bp PCR product using primers lsr-UF/lsr-DR, which amplified upstream and downstream sequences and the chloramphenicol resistance cassette; Lane 3: Negative control; Lane 4: The wild-type strain APEC94 showed a 826-bp PCR product using primers lsrB-F/lsrB-R; Lane 5: The mutant strain APEC94Δlsr(Cm) showed no PCR product using primers lsrB-F/lsrB-R; Lane 6: The lsr complementary strain lsr-APEC94Δlsr showed a 826-bp PCR product using primers lsrB-F/lsrB-R; Lane 7: Negative control.