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. 2019 Dec 12;50:109. doi: 10.1186/s13567-019-0725-0

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© The Author(s) 2019

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Binding of APEC-LsrB to AI-2 using an AI-2 assay. A The activity of APEC-LsrB binding to endogenous AI-2 was evaluated by an AI-2 assay, which showed that the recombinant APEC-LsrB (BL21) bound to endogenous AI-2 produced by wild-type BL21 and released AI-2 at 55 °C. However, the luxS mutant BL21∆luxS did not produce AI-2 so that no AI-2 could be released from APEC-LsrB (BL21∆luxS). B The activity of APEC-LsrB binding to exogenous AI-2 was evaluated by an AI-2 assay, which showed that APEC-LsrB (BL21∆luxS) could bind to exogenous AI-2, resulting in low AI-2 activity in cell-free culture fluid (CF). However, APEC-LsrB (BL21) could not bind to exogenous AI-2 as it was hindered by endogenous AI-2 (produced by wild-type BL21), which resulted in high AI-2 activity in CF.