Table 1.
Bacterial strains and plasmids used in this study
| Strains or plasmid | Relevant genotype and property | Source or references |
|---|---|---|
| Escherichia coli strains | ||
| BL21 | DE3 | Invitrogen |
| BL21ΔluxS | BL21 DE3luxS deletion mutant strain | [23] |
| MG1655 | [16] | |
| DE17 | APEC serotype O2 | [2] |
| DE17ΔluxSΔpfs | DE17 luxS and pfs mutant strain | This study |
| APEC94 | Wide type, isolated from sick chicken (serotype O78) | This study |
| APEC94Δlsr(Cm) | Lacking of lsr operon | This study |
| Salmonella strains | ||
| Salmonella typhimurium | LT2 | ATCC 14028 |
| Vibrio harveyi strains | ||
| BB170 | BB120 luxN::Tn5 (sensor 1−, sensor 2 +) | [13] |
| BB152 | BB120 luxM::Tn5 (representing AI-2) | [21] |
| Plasmids | ||
| pKD46 | The vector containing arabinose-inducible phage λ Red recombinase (AmpR) | [17] |
| pACYC177-lsr | The lsr operon complement plasmid | This study |
| pCold TF | Expression vector with AmpR | TaKaRa |
| pKD3 | The plasmid contained chloramphenicol resistance (CmR) | [17] |
| pCold TF-lsrB | Recombinant plasmid contained lsrB of APEC | This study |