Table 2.
Primers used in this study
| Primers | Oligonucleotide sequence (5′ to 3′) | Description | Product size (bp) |
|---|---|---|---|
| lsrK-F | CTATAACCCAGGCGCTTTCCATA | Partial sequence of lsrK | 1593 |
| lsrK-R | ATGGCTCGACTCTTTACCCTTTC | ||
| lsrR-F | TTAACTACGTAAAATCGCCGCTG | Partial sequence of lsrR | 954 |
| lsrR-R | ATGACAATCAACGATTCGGCAAT | ||
| LsrA-F | ATGCAAACGAGTGATACCCGCGC | Partial sequence of lsrA | 1539 |
| lsrA-R | ACTTCAGCATGACGCCTCCTGAC | ||
| lsrC-F | CTGAAGTTTATTCAGAACAACCGTG | Partial sequence of lsrC | 826 |
| lsrC-R | TATCGATCTGCGTCAGGAACCAT | ||
| lsrD-F | GCCGCATAATGCGTATTCGCTAC | Partial sequence of lsrD | 1003 |
| lsrD-R | CTTTATGGCAATGGGTTATTGGC | ||
| lsrB-F | ATGACACTTCATCGCTTTAAGAa | Partial sequence of lsrB | 826 |
| lsrB-R | CTGAAATTTTGCCTTGCTGAACa | ||
| lsrF-F | GTAAAGATTTTCGTACCGATCAAC | Partial sequence of lsrF | 657 |
| lsrF-R | TTTTACCGCCAGCAATAACAATG | ||
| lsrG-F | ATGCACGTCACACTGGTTGAAAT | Partial sequence of lsrG | 291 |
| lsrG-R | TCACGGCATCAAACCATTGAACA | ||
| APEC94lsr-F | TCCCTTGAATATCGTACTGG | Using for DNA sequencing of lsr operon | |
| APEC94lsr-R | TGCGCTTCGATGTCTTAC | ||
| pCold-F1 | CCCAAGCTTGAAATCGTATTTGCCGAT | Using for identification of lsrB-APEC recombinant plasmid | 1150 |
| pCold-R | GGCAGGGATCTTAGATTCTG | ||
| lsr-UF | TCTAAAAGAAGGGAAATAAGb | The upstream sequence of lsr operon | 763 |
| lsr-UR | CCAGCCTACA GCATGGTACAAGCTGAATC | ||
| lsr-CF | TGTACCATGCTGTAGGCTGGAGCTGCTT | Chloramphenicol resistance cassette (cat) | 1013 |
| lsr-CR | CATTGAACAGCATATGAATATCCTCCTTAGTTC | ||
| lsr-DF | TATTCATATGCTGTTCAATGGGTTGATGC | The downstream sequence of lsr operon | 638 |
| lsr-DR | TGGTGATAGTAGGTGGTTCGb | ||
| PstI-lsr-CF | AAAACTGCAGGGTCTGTATTGAGTGTTAGTTGGAGGTGGGc | The lsr operon complement sequence | 9440 |
| PstI-lsr-CR | AAAACTGCAGCAGTTGCGGATGTCTGCTTc | ||
| LsrB-BamHI-F | CGCGGATCCATGACACTTCATCGCTTTAd | The ORF sequence of lsrB | 1023 |
| LsrB-HindIII-R | CCACTTTCAACGAGCTGATGe | ||
| fimH-RTF | GTGCCAATTCCTCTTACCGTT | Partial DNA sequence of fimbrial protein FimH | 165 |
| fimH-RTR | TGGAATAATCGTACCGTTGCG | ||
| fliD-RTF | TTCAGACGCAGTTGAAATCG | Partial DNA sequence of flagellar filament capping protein FliD | 184 |
| fliD-RTR | TTACCATCGCCAACAATCAA | ||
| flhA-RTF | CGGCATCGTACTCTGGAACT | Partial DNA sequence of flagellar biosynthesis gene flhA | 172 |
| flhA-RTR | CATTACCCATCCGTTCGTTC | ||
| dosP-RTF | ATAAACCCACGCCCATATCA | Partial DNA sequence of oxygen-sensing cyclic-di-GMP phosphodiesterase DosP | 194 |
| dosP-RTR | GGCGTTATCCGTGAACTTGT | ||
| pgaA-RTF | GGCAATGGTCTCCTTGTGAT | Partial DNA sequence of poly-beta-1,6 N-acetyl-d-glucosamine export porin PgaA | 195 |
| pgaA-RTR | GATCATCTTGGCGCGTTATT | ||
| fhuD-RTF | AACTATCGCCTGTGGGTCAG | Partial DNA sequence of iron-hydroxamate transporter substrate-binding subunit fhuD | 153 |
| fhuD-RTR | TGCCAGCATTTCTGATGAAG | ||
| dnaE-RTF | GATTGAGCGTTATGTCGGAGGC | Partial DNA sequence of the internal control dnaE | 80 |
| dnaE-RTR | GCCCCGCAGCCGTGAT |
aA 826-bp PCR product was amplified from wild-type strain APEC94 but no PCR product was obtained from mutant strain APEC94Δlsr(Cm) using primers lsrB-F/lsrB-R.
bA 2414-bp PCR product was amplified from mutant strain APEC94Δlsr(Cm) but no product was amplified from wild-type strain APEC94 in a limited amplification time using primers lsr-UF/lsr-DR.
cPstI restriction sites are underlined.
dBamHI restriction sites are underlined.
eHindIII restriction sites are underlined.