Skip to main content
. 2019 Nov 18;19(1):367–374. doi: 10.3892/etm.2019.8207

Figure 1.

Figure 1.

MAT inhibits MDA-MB-231 and MCF-7 cell growth by inducing cell apoptosis. Following incubation with various concentrations of MAT (0–4 mg/ml) for 24 and 48 h, (A) MDA-MB-231 and (B) MCF-7 cell proliferation was measured by MTT assay. (C) MDA-MB-231 and (D) MCF-7 cells were treated with or without MAT (2 mg/ml) for 48 h and stained with Annexin V (5 µg/ml)/PI (10 µg/ml) prior to being analyzed by flow cytometry. Cells labeled with Annexin V(−) PI(+) are shown in the Q1 area, cells labeled with Annexin V(+) PI(+) in the Q2 area, cells labeled with Annexin V(−) PI(−) in the Q3 area and cells labeled with Annexin V(+) PI(−) in the Q4 area. *P<0.05, **P<0.01 and ***P<0.001 MAT vs. CTL by one-way analysis of variance followed by Student-Newman-Keuls post hoc test. MAT, matrine; NC, negative control; PI, propidium iodide; MAT, matrine; FITC, fluorescein isothiocyanate; CTL, control.