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. 2019 Dec 12;19:552. doi: 10.1186/s12870-019-2174-3

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© The Author(s). 2019

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 5 — Localization of MsLAC1 in the cell wall compartment after transient expression in Nicotiana benthamiana leaf. Transient expression of MsLAC1-mCherry and LT16b-GFP (plasma membrane marker fused with GFP) in tobacco epidermal leaf cells after co-infiltration with Agrobacteria harboring the respective constructs. Upper panel shows cell wall association of MsLAC1, whereas the Lti6-GFP-labelled plasma membrane retracts. Lower panel shows a cell co-expressing Lti6b-GFP and a cytosolic mCherry control retracting with the protoplast. All pictures were taken 2 h after onset of plasmolysis (i.e. incubation in 30% w/v sucrose solution). Scale bars: 16 μm