Fig. 4.

FXR suppressed gluconeogenesis and enhanced glucose uptake in the HK-2 cell lines. a, b The protein expression of FXR was obviously upregulated by GW4064 at 24, 48 and 72 h in the HK-2 cell lines. GW4064 at 4 µM inhibited the mRNA level of FXR (c) and promoted the mRNA expression of SHP1 (d) and GLUT2 (f) at the same time. e The mRNA level of PEPCK was obviously inhibited by GW4064 treatment for 24, 48 and 72 h. g The mRNA expression of FXR was inhibited by si-FXR at 50 nM. The mRNA expression of SHP-1 (h) and GLUT2 (i) were obviously inhibited when FXR was knocked out and the PEPCK (j) mRNA expression level was promoted at the same time. Data are presented as the mean ± SD (n = 3 per group). *P < 0.05 vs. the control group