Fig. 5.

Dynamic expression of CXCR3-B during tumor cells dissemination. a Schematic of intrasplenic injection model with spontaneous metastasis to the liver. b Representative spleen and liver images and H&E staining with or without tumors after PCa cells inoculation (mice N = 6). Bar = 250 μm. c Representative IHC staining of CXCR3-B in splenic and hepatic tumors. Tumor area is outlined and pointed by black arrow. Bar = 50 μm. d Representative of IHC staining of CXCR3-B in different size of hepatic tumor nodules, all from the same liver section, tumor area is outlined. The correlation of tumor nodules area and semi-quantitative CXCR3-B expression levels is on the right. Bar = 50 μm. Nonlinear fit line with R-squared value has been shown