Figure 5.

Effect of miR-144-5p overexpression on NK-κB activation. Cells transfected with miR144-5p mimic or mimic control were stimulated with LPS for 24 h, following which western blotting was performed to measure the protein levels of TLR2, p-p65, and p65. (A) Representative western blot images of TLR2 expression and p65 phosphorylation. (B) Quantified densitometry results. (C) The mRNA level of TLR2 was detected using reverse transcription-quantitative PCR. Data are presented as the mean ± SD. **P<0.01 vs. NC group. miR, microRNA; LPS, lipopolysaccharide; NC, mimic negative control; TLR2, toll-like receptor 2; p-p65, phosphorylated p65.