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. 2019 May 15;30(5):544–555. doi: 10.1089/hum.2018.079

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Copyright 2019, Mary Ann Liebert, Inc., publishers

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Figure 1. — Green fluorescent protein (GFP) expression across the mouse nervous system after intrathecal osmotic pump delivery, and cell types transduced. (a) Dose-dependent rAAV9-CBA-GFP expression in the spinal cord after intrathecal osmotic pump (IT pump) delivery at 1.5 × 10¹¹ and 3 × 10¹¹ genome copies (GC). (b) Cell types targeted in the spinal cord after IT pump delivery identified by costaining with various cellular markers including GFAP (glial fibrillary acidic protein; astrocytes), IBA1 (ionized calcium binding adapter molecule 1; microglia), CD31 (endothelial cells), NeuN (neuronal nuclei; neurons), Olig2 (oligodendrocyte transcription factor 2; oligodendrocytes), and SMA (α-smooth muscle actin; smooth muscle cells). (c) Dose-dependent rAAV9-CBA-GFP expression in the brain after IT pump delivery at 1.5 × 10¹¹ and 3 × 10¹¹ GC. (d) Cell types targeted in the brain after IT delivery, identified by costaining with various cellular markers. Thick short-stemmed white arrow indicates a neuron targeted. Blue arrows indicate where the image is taken from brain anatomy. (e) Dose-dependent rAAV9-CBA-GFP expression in dorsal root ganglia (DRG) seen in various regions after IT pump delivery at 1.5 × 10¹¹ and 3 × 10¹¹ GC, as well as cell types targeted. These are identified by costaining with neuronal markers NeuN and neurofilament (NF). Thin long-stemmed white arrows in each panel indicate co-stained cells. PBS, phosphate-buffered saline. Color images are available online.

Figure 1. — Green fluorescent protein (GFP) expression across the mouse nervous system after intrathecal osmotic pump delivery, and cell types transduced. (a) Dose-dependent rAAV9-CBA-GFP expression in the spinal cord after intrathecal osmotic pump (IT pump) delivery at 1.5 × 10¹¹ and 3 × 10¹¹ genome copies (GC). (b) Cell types targeted in the spinal cord after IT pump delivery identified by costaining with various cellular markers including GFAP (glial fibrillary acidic protein; astrocytes), IBA1 (ionized calcium binding adapter molecule 1; microglia), CD31 (endothelial cells), NeuN (neuronal nuclei; neurons), Olig2 (oligodendrocyte transcription factor 2; oligodendrocytes), and SMA (α-smooth muscle actin; smooth muscle cells). (c) Dose-dependent rAAV9-CBA-GFP expression in the brain after IT pump delivery at 1.5 × 10¹¹ and 3 × 10¹¹ GC. (d) Cell types targeted in the brain after IT delivery, identified by costaining with various cellular markers. Thick short-stemmed white arrow indicates a neuron targeted. Blue arrows indicate where the image is taken from brain anatomy. (e) Dose-dependent rAAV9-CBA-GFP expression in dorsal root ganglia (DRG) seen in various regions after IT pump delivery at 1.5 × 10¹¹ and 3 × 10¹¹ GC, as well as cell types targeted. These are identified by costaining with neuronal markers NeuN and neurofilament (NF). Thin long-stemmed white arrows in each panel indicate co-stained cells. PBS, phosphate-buffered saline. Color images are available online.