Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2019 May 15;30(5):544–555. doi: 10.1089/hum.2018.079

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright 2019, Mary Ann Liebert, Inc., publishers

PMC Copyright notice

Figure 3. — Distribution and cell types immunoreactive for HA-tagged ABCD1 after intrathecal delivery of rAAV9-CBA-hABCD1-HA. (a) Colocalization of HA tag with ABCD1 after rAAV9-CBA-hABCD1-HA transduction at 5 × 10⁵ GC/cell in mixed glial cell culture. (b) Representative images show rAAV9-CBA-hABCD1-HA distribution across the whole spinal cord after IT pump and IT bolus delivery at 2 × 10¹¹ GC/mouse. Higher magnification image shows punctate HA tag expression (HA, green) in glial cells and some neurons (NF, red). (c) Representative images show rAAV9-CBA-hABCD1-HA distribution across DRGs after IT pump and IT bolus delivery at 2 × 10¹¹ GC/mouse. Higher magnification image shows punctate HA tag expression (HA, green) in DRG neurons (NF, red). (d) Cell types targeted in the spinal cord after IT delivery identified by costaining with various cellular markers including GFAP (astrocytes), IBA1 (microglia), NeuN (neurons), and Olig2 (oligodendrocytes) (HA, red). (e) Representative images show rAAV9-CBA-hABCD1-HA distribution in peripheral organs after IT pump and IT bolus delivery at 2 × 10¹¹ GC/mouse. ABCD1, peroxisomal ATP-binding cassette transporter subfamily D member 1; DRG, dorsal root ganglia; GC, genome copies; GFAP, glial fibrillary acidic protein; HA, hemagglutinin; IBA1, ionized calcium binding adapter molecule 1; IT, intrathecal; NeuN, neural nuclei; NF, neurofilament; Olig2, oligodendrocyte transcription factor 2; SC, spinal cord. Color images are available online.

Figure 3. — Distribution and cell types immunoreactive for HA-tagged ABCD1 after intrathecal delivery of rAAV9-CBA-hABCD1-HA. (a) Colocalization of HA tag with ABCD1 after rAAV9-CBA-hABCD1-HA transduction at 5 × 10⁵ GC/cell in mixed glial cell culture. (b) Representative images show rAAV9-CBA-hABCD1-HA distribution across the whole spinal cord after IT pump and IT bolus delivery at 2 × 10¹¹ GC/mouse. Higher magnification image shows punctate HA tag expression (HA, green) in glial cells and some neurons (NF, red). (c) Representative images show rAAV9-CBA-hABCD1-HA distribution across DRGs after IT pump and IT bolus delivery at 2 × 10¹¹ GC/mouse. Higher magnification image shows punctate HA tag expression (HA, green) in DRG neurons (NF, red). (d) Cell types targeted in the spinal cord after IT delivery identified by costaining with various cellular markers including GFAP (astrocytes), IBA1 (microglia), NeuN (neurons), and Olig2 (oligodendrocytes) (HA, red). (e) Representative images show rAAV9-CBA-hABCD1-HA distribution in peripheral organs after IT pump and IT bolus delivery at 2 × 10¹¹ GC/mouse. ABCD1, peroxisomal ATP-binding cassette transporter subfamily D member 1; DRG, dorsal root ganglia; GC, genome copies; GFAP, glial fibrillary acidic protein; HA, hemagglutinin; IBA1, ionized calcium binding adapter molecule 1; IT, intrathecal; NeuN, neural nuclei; NF, neurofilament; Olig2, oligodendrocyte transcription factor 2; SC, spinal cord. Color images are available online.