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. 2019 Nov 18;19(1):205–213. doi: 10.3892/etm.2019.8209

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Copyright: © Yu et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 3. — Representative micrographs used for the counting of the ERRγ positive motor neurons on both sides of the same C7 segment of the spinal cord. (A) The contralateral side at 2 weeks. (B) The ipsilateral side at 2 weeks. (C) The ipsilateral side at 4 weeks. (D) The contralateral side at 4 weeks; all at magnification ×20) In the ERRγ-stained sections, the numbers of positive motor neurons were notably decreased on the ipsilateral side compared with those on the contralateral side. Red arrows show positive ERR γ signals in smaller γ motor neurons; Green arrows depict positive ERR γ signals in large α motor neurons. (E) The mean of the total number of ERRγ-positive motor neurons in 10 serial sections of both ventral horns of C7 spinal segments were computed at 2 and 4 weeks post-avulsion. The average number of ERRγ-positive motor cells on the injured side (black column, ipsilateral) were significantly decreased compared with that identified on the contralateral side (gray column, contralateral). *P<0.05. ERRγ, estrogen-related receptor γ.