Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2019 Nov 18;19(1):205–213. doi: 10.3892/etm.2019.8209

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright: © Yu et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

PMC Copyright notice

Figure 4. — Complementary expression of ERRγ and NeuN in γ and α motor neurons. Immunofluorescence double-labeling reaction results indicated that the positive immunoreactions at (A) 2 weeks in the contralateral side of the spine, (B) 2 weeks in the ipsilateral side of the spine, (C) 4 weeks in the ipsilateral side of the spine and (D) 4 weeks in the contralateral side of the spine. ERRγ (red) were located in the cytoplasm of the spinal neurons. The figures also show the apparent downregulation of the ERRγ signal on the injured ipsilateral sides at the time points after avulsion. NeuN (green) signals were concentrated in the nuclei of motor neurons as shown after (E) 2 weeks in the contralateral side of the spine, (F) 2 weeks in the ipsilateral side of the spine, (G) 4 weeks in the ipsilateral side of the spine and (H) 4 weeks in the contralateral side of the spine. Representative micrographs after (I) 2 weeks in the contralateral side of the spine, (J) 2 weeks in the ipsilateral side of the spine, (K) 4 weeks in the ipsilateral side of the spine and (L) 4 weeks in the contralateral side of the spine show that the bulk all of the ERR γ-positive motor neurons were also NeuN-positive motor cells (ERRγ^on/NeuN^on). However, the remainder of these ventral motor neurons were ERRγ^on/NeuN^off. (M) ERRγ was detected in both populations of motor neurons ERRγ^on/NeuN^off status of motor neurons can be identified as γ motor neurons. ERRγ, estrogen-related receptor γ; NeuN, RNA binding protein fox-1 homolog 3.