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. 2019 Dec 6;9:1379. doi: 10.3389/fonc.2019.01379

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Copyright © 2019 Lin, Chang, Cheng and Liu.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Association of SMAD4 knockdown with oncogenic phenotypes in HNSCC cells. (A) Western blotting for SMAD4 expression in NOK cells and the seven HNSCC cell lines investigated. In SAS and SCC25 cells, SMAD4 expression levels are higher compared to other cell lines. (B) Western blotting on extracts from sh-Luc cell subclone (control) and sh-SMA1D4 (321) cell subclone, which was established from the construct TRCN0000010321. (C–E) SAS and (F–H) SCC25 cells: proliferation (C,F, respectively), migration (D,G, respectively), and invasion (E,H, respectively).