FIG. 1.

Changes in glomerular phenotypes in db/m and db/db mice with or without hEC-SOD treatment. Glomerular mesangial fractional area, tubulointerstitial fibrosis, and TGF-β1 and type IV collagen expression in the glomerulus in the cortical area of db/m and db/db mice with or without hEC-SOD treatment are shown. (A) Representative sections of periodic acid-Schiff stain, Masson's trichrome staining and representative immunohistochemical staining for TGF-β1 and type IV collagen are shown (original magnification, × 400). Quantitative analyses of the results for the (B) mesangial fractional area (%), (C) interstitial fibrosis area (%), (D) TGF-β1 (fold), and (E) type IV collagen (fold) are shown. **p < 0.01 and ^#p < 0.001 vs. db/m (db/m cont), dm cont (db/m cont), dm+hEC-SOD (db/m hEC-SOD) and db+hEC-SOD (db/db hEC-SOD) mice. hEC-SOD, human recombinant extracellular superoxide dismutase; TGF-β1, transforming growth factor-β1. To see this illustration in color, the reader is referred to the web version of this article at www.liebertpub.com/ars