FIG. 2.

Western blot analysis of the intrarenal SOD isoforms and immunofluorescence staining for SOD3 in db/m and db/db mice with or without hEC-SOD treatment. Protein lysates (30 μg) from renal cortex were separated by SDS-PAGE and analyzed by Western blotting. (A) Representative Western blot is shown for SOD1, SOD2, SOD3, and β-actin. Quantitative analyses are shown for (Supplementary Fig. S1; Supplementary Data are available online at www.liebertpub.com/ars). (B) SOD1/β-actin and (C) SOD2/β-actin, ^#p < 0.001 vs. dm cont (db/m cont) and dm+hEC-SOD (db/m hEC-SOD) mice. (D) SOD3/β-actin. **p < 0.01 vs. dm cont (db/m cont), dm+hEC-SOD (db/m hEC-SOD) and db+hEC-SOD (db/db hEC-SOD) mice. (E) Representative immunofluorescence staining for SOD3 is shown in db/m and db/db mice with or without hEC-SOD treatment. SDS-PAGE, sodium dodecyl sulfate-polyacrylamide gel electrophoresis. To see this illustration in color, the reader is referred to the web version of this article at www.liebertpub.com/ars