Figure 4.

CS extract enhances the expression of filaggrin in skin of NC/Nga mice. (A) For filaggrin analysis, skin sections were fixed, embedded in paraffin, and stained with immunohistochemical stains. The samples were examined by using light microscopy (magnification, ×100). (B) Filaggrin, loricrin, and involucrin as well as phospho-JNK, in the dorsal skin were analyzed by western blotting. Densitometric data are expressed as means ± SD and are presented relative to the negative control, which was set at 1 (lower panels of B and C). *P < 0.05 and **P < 0.01 indicate a significant difference between the untreated and control groups or between the control and CS-treated groups.