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. 2019 Oct 21;16(12):1593–1603. doi: 10.7150/ijms.37677

Figure 2.

Figure 2

(A) Negative control siRNA (N.C. siRNA) or PPAR δ siRNA-transfected L02 cells were incubated in 0.4mM palmitic acid or (and) 800ng/ml LPS for 24h. (a) Relative expression level of IRS-1, PI3K, AKT and p-AKT were determined by Western blotting. (b-e) represent relative expression levels of IRS-1, PI3K, AKT and p-AKT. (B) L02 cells were exposed to 0.4mM palmitic acid or (and) 800ng/ml LPS with or without GW0742 treatment. (a) Relative expression level of IRS-1, PI3K, AKT and p-AKT were determined by Western blotting. (b-e) represent relative expression levels of IRS-1, PI3K, AKT and p-AKT. PA, palmitic acid. GW, GW0742.