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. 2019 Aug 12;14(4):041004. doi: 10.1116/1.5102150

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Copyright © 2019 Author(s)

Published by the AVS.

1934-8630/2019/14(4)/041004/13/$30.00

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Fig. 8. — Gene expression in sACL fibroblasts over time in the presence of the PCL films. Samples were processed for total cellular RNA extraction, cDNA synthesis, and real-time RT-PCR amplification. NG: nongrafted films; G: grafted films. The genes analyzed included type-I collagen [(a) and (d)], type-III collagen [(b) and (e)], and TNF-α [(c) and (f)], with GAPDH serving as a housekeeping gene and internal control. Ct values were obtained for each target and GAPDH as a control for normalization, and fold inductions (relative to cells maintained in absence of films) were measured using the 2⁻^ΔΔ^Ct method (*p ≤ 0.05 and ** p ≤ 0.005).