Table 1.
Photophysical features of typical fluorescent lifetime organic dyes.
|
Types of organic dyes |
Organic dyes | Solvents | Photophysical features | References | |||
|---|---|---|---|---|---|---|---|
|
Absorption wavelength Abs (nm) |
Emission wavelength Em (nm) |
Lift time (in vitro) (ns) |
Lift time (in vivo) (ns) |
||||
| Cyanine | Cy 5 | H2O | 651 | 670 | 0.98 | – | Tinnefeld et al., 2001 |
| Cy 5.5 | – | 675 | 694 | – | – | Martinić et al., 2017 | |
| Cy 7 | – | 755 | 788 | – | – | Martinić et al., 2017 | |
| Cy 7.5 | – | 788 | 808 | – | – | Martinić et al., 2017 | |
| CyNA-414 | – | 804 | 819 | – | – | Samanta et al., 2010 | |
| ICG | DMSO | 794 | 831 | 1.11 | 0.60–0.84 | Berezin et al., 2009a | |
| D1 | Methanol | 786 | 814 | – | – | Chen et al., 2006 | |
| D2 | Methanol | 785 | 813 | – | – | Chen et al., 2006 | |
| D3 | Methanol | 784 | 809 | – | – | Chen et al., 2006 | |
| D4 | Methanol | 785 | 807 | – | – | Chen et al., 2006 | |
| D5 | Methanol | 784 | 813 | – | – | Chen et al., 2006 | |
| E | Methanol | 782 | 808 | – | – | Chen et al., 2006 | |
| N-CyNp | HEPES | – | 785 | – | – | Wu et al., 2017 | |
| pyrrolopyrrole cyanine-BF2 (PPC-BF2) |
DMSO | 757 | 779 | 4.02 | 3.05–3.80 | Berezin et al., 2009a | |
| pyrrolopyrrole cyanine-BPh2 (PPC-BPh2) |
DMSO | 824 | 840 | 3.35 | 2.50–2.88 | Berezin et al., 2009a | |
| LS-288 | Methanol | 770 | 790 | 0.81 | 1.12 | Berezin et al., 2009b | |
| DMSO | Methanol | 782 | 810 | 0.76 | – | Waddell et al., 2000 | |
| LS-277 | Methanol | 800 | 811 | 0.61 | 0.74 | Berezin et al., 2009b | |
| IR-820 | Methanol | 820 | 836 | 0.25 | 0.53 | Berezin et al., 2009b | |
| LS-276 | Methanol | 797 | 816 | 0.83 | 1.12 | Berezin et al., 2009b | |
| Cypate | Methanol | 792 | 817 | 0.46 | 0.63 | Berezin et al., 2009b | |
| Squaraine | N-propanesulfonate-benzothiazolium squaraine (SQ-1) | Aqueous Solution (BSA) |
660 ±1 | 669 ±1 | 3.7 ± 0.2 | – | Zhang et al., 2013 |
| N-propanesulfonate-benzoindolium squaraine (SQ-2) | Aqueous Solution (BSA) |
675 ± 1 | 684 ± 1 | 2.6 ± 0.1 | – | Zhang et al., 2013 | |
| Squaraine Dye 1 | DMSO | 706 ± 1 | 719 ± 1 | 0.51 ± 0.07 | – | Ahn et al., 2012 | |
| Q1 | THF:water (4:1) | 650 | 676 | 1.24 | – | Arunkumar et al., 2005 | |
| Q2 | THF:water (4:1) | 643 | 667 | 6.96 | – | Arunkumar et al., 2005 | |
| Rhodamine dyes | Rhodamine 110 | Water | 497 | 523 | 4.28 | – | Zhang X.-F. et al., 2014 |
| Rhodamine 123 | Water | 497 | 527 | 4.20 | – | Zhang X.-F. et al., 2014 | |
| Rhodamine 6G | Water | 525 | 554 | 4.22 | – | Zhang X.-F. et al., 2014 | |
| Rhodamine B | Water | 554 | 580 | 1.75 | – | Zhang X.-F. et al., 2014 | |
| Ethyl-RB | Water | 552 | 581 | 1.10 | – | Zhang X.-F. et al., 2014 | |
| Butyl-RB | Water | 558 | 584 | 1.67 | – | Zhang X.-F. et al., 2014 | |
| Rhodamine 101 | Water | 575 | 604 | 4.91 | – | Zhang X.-F. et al., 2014 | |
| LA-RB | Water | 562 | 588 | 1.49 | – | Zhang X.-F. et al., 2014 | |
| Sulfo-RB | Water | 554 | 591 | 1.78 | – | Zhang X.-F. et al., 2014 | |
| BODIPYs | BODIPY dye 1 | Hexane | 690 | 693 | 4.49 | – | Sun et al., 2019 |
| BODIPY dye 2 | Hexane | 714 | 716 | 3.89 | – | Sun et al., 2019 | |
| BODIPY dye A | H2O | 622/665 | 702 | 2.6 | – | Zhu et al., 2013 | |
| Polymer A | H2O | 687 | 711 | 0.4 | – | Zhu et al., 2013 | |
| Polymer B | H2O | 689 | 712 | 0.6 | – | Zhu et al., 2013 | |