Table 2.
Main Effects of Rapamycin on All Mice Tested
| Term glucose (mg/dL) | Term insulin (ng/mL) | GTT | ITT | |||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 0 (mg/dL) | 15 (mg/dL) | 120 (mg/dL) | AUC (×1,000) | 0–15 (mg/dL) | 15–120 (mg/dL) | 0 (mg/dL) | 60 (mg/dL) | AUC raw (×1,000) | AUC % (×1,000) | |||
| Strain | <.0001 | <.0001 | <.0001 | <.0001 | <.0001 | <.0001 | <.0001 | <.0001 | <.0001 | <.0001 | <.0001 | <.0001 |
| Treat | <.0001 | n.s. | n.s. | <.0001 | <.0001 | <.0001 | <.0001 | n.s. | =.0003 | n.s. | =.02 | n.s. |
| Treat × strain | =.0003 | n.s. | <.0001 | <.0001 | <.0001 | <.0001 | <.0001 | <.0001 | n.s. | =.06 | =.07 | n.s. |
| UNT | 158 ± 8 | 1.5 ± .3 | 90 ± 7 | 236 ± 9 | 137 ± 12 | 21.8 ± 1.2 | 147 ± 10 | 99 ± 10 | 130 ± 4 | 107 ± 5 | 6.7 ± 0.2 | 5.2 ± 0.1 |
| RAPA | 209 ± 7 | 1.4 ± .3 | 91 ± 6 | 306 ± 10 | 210 ± 12 | 30.1 ± 1.2 | 215 ± 9 | 96 ± 9 | 145 ± 4 | 108 ± 5 | 7.2 ± 0.2 | 5.0 ± 0.1 |
Note: Values are mean ± SEM. Rapamycin has no effect on overnight-fasted glucose (GTT-0) but does increase morning-fasted glucose (ITT-0). Rapamycin increases the glucose value at the 15-minute time point of the GTT but has no effect on the amount of glucose cleared from 15 to 120 minutes. Area under the curve (AUC) for ITT is calculated using both the absolute PG values as well as using the percentage of the baseline value. Lack of significance for AUC % indicates the significant effect seen for the raw AUC is driven by the significant elevation in the ITT-0 time point value. n = 49–50 (untreated), n = 54 (RAPA-treated). Cohorts 2–4 for B6 and cohort 2 for B6N not included to keep contribution of each strain balanced. AUC = area under curve; GTT = glucose tolerance test; ITT = insulin tolerance test; RAPA = rapamycin; UNT = untreated.