Orai1 promotes the metastasis of osteosarcoma cells by activating the Rac1-WAVE2 signaling pathway. (A) The expression levels of phosphorylated Rac1 (p-Rac1), Rac1, phosphorylated WAVE2 (p-WAVE2) and WAVE2 were detected in MG-63 cells which were silenced by small interfering RNAs against Orai1. (B) After incubation with NSC33788, the amount of cell migration was detected in MG-63 cells that were silenced by small interfering RNAs against Orai1. (C) After incubated with NSC33788, the amount of cell invasion was detected in MG-63 cells which were silenced by small interfering RNAs against Orai1. (D) After incubation with NSC33788, the amount of cell adhesion was detected in MG-63 cells that were silenced by small interfering RNAs against Orai1. Scale=100 μm.