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. 2019 Oct 23;15(13):2763–2773. doi: 10.7150/ijbs.38859

Figure 1.

Figure 1

Deletion of PRMT1 results in anemia and leukopenia. (A) Relative Prmt1 mRNA levels in indicated HSPCs populations, detected by quantitative reverse transcriptase polymerase chain reaction (qRT-PCR; n=3, each performed in duplicate).*p<0.05, p value was determined by one way ANOVA. (B) Generation of a PRMT1 flox allele in mice. Representation of the floxed exons are the blue boxes, and the line represents introns. The black triangles denote loxP sites, and the small arrows denote the primers used for PCR analysis. The PRMT1 conditional allele consists of exons 4 and 5, which encode part of the methyltransferase domain, flanked by loxP sites. (C) Genomic DNA was purified from tails for further PCR analysis. Tissue from 3 different PRMT1f/f and PRMT1f/f/Mx1-CRE mice was used (n=3). Performed in duplicate from 3 independent experiments. (D) PRMT1 protein levels were determined by Western blot analysis using PRMT1f/f or PRMT1f/f/Mx1-CRE mouse BM cells (n=3). Performed in duplicate from 3 independent experiments. (E) Complete blood count (CBC) analysis of peripheral blood cell RBC, Hb, WBC, neutrophil, lymphocyte and Plt counts at 12 weeks after pIpC injection are shown(n=10).**p<0.01,***p<0.001. p values were determined by Mann-Whitney U tests. (F) Number of total BM and spleen cells from PRMT1f/f and PRMT1f/f/Mx1-CRE mice (n=10). ***p<0.001. p value was determined by Mann-Whitney U tests. (G) Representative H&E- staining image shows cross sections of femurs isolated from the PRMT1f/f and PRMT1f/f/Mx1-CRE mice (scale bars, 100µm).