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. 2019 Oct 23;15(13):2763–2773. doi: 10.7150/ijbs.38859

Figure 3.

Figure 3

PRMT1 deletion results in adult mouse HSPCs reduction. (A) Representative FACS plots showing gating strategy and frequency of phenotypic populations including LSKs, MPs, CMPs, GMPs and MEPs from PRMT1f/f/Mx1-CRE and of PRMT1f/f mouse BM. (B) Frequency of LSK, LT-HSC, ST-HSC, MPP, LMPP and CLP populations in PRMT1f/f/Mx1-CRE and of PRMT1f/f mouse BM (n=10). *p<0.05. p value was determined by Student's t test. (C) Frequency of CMP, GMP and MEP subsets in PRMT1f/f/Mx1-CRE and of PRMT1f/f mouse BM (n=10).**p<0.01. p value was determined by Student's t test. (D) Number of LSK, LT-HSC, ST-HSC, MPP, LMPP and CLP populations in PRMT1f/f/Mx1-CRE and of PRMT1f/f mouse BM (n=10). **p<0.01. p value was determined by Mann-Whitney U tests. (E) Number of CMP, GMP and MEP subsets in PRMT1f/f/Mx1-CRE and of PRMT1f/f mouse BM (n=10). ***p<0.001. p value was determined by Mann-Whitney U tests. (F) Colony-forming progenitor (CFU) assays were performed using PRMT1f/f/Mx1-CRE and PRMT1f/f mouse bone marrow cells. CFU- number was shown. Cells were replated over 2 rounds of weekly successive replating (P2, P3). Performed in duplicate from 3 independent experiments.*p<0.05, **p<0.01. p values were determined by Student's test. (G) Percentage of BFU-E, CFU-GM, CFU-GEMM, CFU-G and CFU-M colonies at 1st plating. *p<0.05. Performed in duplicate from 3 independent experiments. p value was determined by Student's test.