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. 2019 Nov 8;15(13):2859–2871. doi: 10.7150/ijbs.39063

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Triple-negative breast cancer (TNBC) cells can alter the cytoskeleton of macrophages. (A) White lysis zone indicates MMP-9 activity in gelatin zymography. (B) The adhesion ability of macrophages treated with different CM were not significant. P>0.05. (C) Quantitative polymerase chain reaction (qPCR) data showing the mRNA expression of Itgam and Icam in macrophages treated with different CM. P>0.05. (D) qPCR data showing the mRNA expression of Capza1 and Cfl1 in macrophages treated with different CM. *P<0.05. (E) The cells that passed through the membrane in the Transwell migratory assay were imaged using a confocal microscope. Scale bar, 50 μm. (F) Green staining indicates F-actin and blue indicates nuclei in immunofluorescence staining. Scale bar, 10 μm. (G) The height of the peak represents the amount of F-actin in the macrophages.