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. 2019 Nov 8;15(13):2859–2871. doi: 10.7150/ijbs.39063

Figure 4.

Figure 4

Detection of macrophage colony-stimulating factor (M-CSF) expression and secretion in different breast cancer cells. (A) These bars were sorted by combined score which is computed by taking the log of the P-value from the Fisher exact test and multiplying that by the Z-score of the deviation from the expected rank. (B) Enriched biological processes are the columns, input genes are the rows, and cells in the matrix indicate if a gene is associated with a process. (C) The GDS4061 dataset showed that the expression of M-CSF in the ER knockout MCF-7 cells was significantly higher than that in the control. P<0.05. (D) The detection of ER and M-CSF in paraffin-embedded breast cancer specimens. Brown staining denotes the immunoreactivity of ER or M-CSF. The tumor sections were counterstained by Hematoxylin to label the nuclei. Scale bar, 50 µm. (E) Western blotting revealed that the M-CSF level in the HCC1937 cell or MDA-MB-231 cell was higher than that in the MCF-7 cell. (F) ELISA showed that the secreted M-CSF level in the HCC1937 CM or MDA-MB-231 CM was higher than that in the MCF-7 CM.