Fig. 5.

LSD1 inhibition prevented the normal decrease in expression of progenitor genes. a Selected top IPA canonical pathways enriched with genes that were significantly upregulated at retina explants after LSD1 inhibitor treatment for 8 days compared to untreated control. Data from the expression microarray were analyzed by IPA’s pathway enrichment functionality. p value showed –log on y-axis and the line represents –log 2.0=p<0.01. List of pathways: 1. Axonal guidance signaling; 2. Cell cycle signaling; 3. Cxrc signaling; 4. Wnt signaling; 5. Phospholipase C signaling; 6. Nanog signaling; 7. Protein kinase A signaling; 8. Notch signaling. b IPA gene network analysis related to STAT3 signaling. Genes in close boxes represented upregulated expression in retina explant cultures following LSD1 inhibitor treatment compared to their untreated controls (Genes are listed in Online Resource table 3.2 under network “Cell-To-Cell Signaling and Interaction, Inflammatory Response, Cell Cycle”). c IPA gene causal network analysis related to HES1 signaling. Genes in close boxes represented upregulated expression in retina explant cultures following LSD1 inhibitor treatment compared to their untreated controls (Genes are listed in Online Resource table 3.2 under network “Nervous System Development and Function, Tissue Morphology, Visual System Development and Function”). Enlarged version of B and C is in Online Resource Figs. 1 and 2