Fig. 8.

LSD1 binding to genomic loci of selected genes upregulated by inhibition of LSD1. a–d LSD1 accumulation on loci of genes maintained after LSD1 inhibition in PN1 and PN28 retinal explants. Quantitative real-time PCRs after LSD1 ChIP were performed with primers specific for Gnat2 (a), Smad3 (b), Hes1 (c), and Rho (d) genes (Online Resource Table 1). All primer pairs for Gnat2 and Smad3 have NR-binding sites inside PCR amplified regions. e Hes1 genomic loci with primer pairs for Hes1 gene correspond to following regions: 1, 3, and 4 have no NR-binding motif; 2, 5, 6 have NR-binding sites inside or in closed proximity to PCR amplified regions. Dotted lines around primers 5 and 6 represent length of sonicated DNA fragments from chromatin immunoprecipitation that could be tested by these primers. DNAse Hypersensitive Sites (DHS) at 3 developmental stages at the Hes1 locus are taken from ENCODE for reference. Experiments done in three technical replicates, *p=0.016; **p<0.09; ***p=0.002