Fig. 9.

LSD1 inhibition increased gene expression in other retinal cell types. a Confirmation of microarray data for examples of bipolar and cone-specific genes by RT-PCR. RNA in 3 biological replicas was purified from retinal explants cultured for 8 days with TCP or control. After cDNA isolation RT-PCRs were done with gene’s specific primers (Online Resource Table 1). b Amount of GNAT2 protein is increased in PN1 retinal explants cultured for 8 days in the presence of LSD1 inhibitors, TCP. Western blot of explant culture samples probed with antibody against GNAT2 with Coomassie staining of core histones as loading control. Quantification of GNAT2 band intensities normalized to histone loading, **p<0.001; n=3