Figure 2.

Overexpressed MAPK10 leads to inhibited proliferation, migration and invasion of cervical cancer cells. SiHa cells are transfected with oe-MAPK10 and oe-NC. (A), The mRNA expression of MAPK10, the AP-1 family members (c-FOS, c-JUN and JUNB) and angiogenesis related factor VEGF detected by RT-qPCR. (B), The protein expression of MAPK10, the AP-1 family members (c-FOS, c-JUN and JUNB) and angiogenesis related factor VEGF measured by Western blot analysis. (C), Cell viability (× 200) assessed by EdU assay. (D), Cell invasion ability (× 200) determined by Transwell assay. (E), Cell migration ability (× 200) identified by Transwell assay. *p < 0.05 vs SiHa cells transfected with oe-NC. The above data are all documented measurement data. Comparisons between two groups were analyzed by non-paired t-test. The experiments are repeated 3 times independently.

Abbreviations: EdU, 5-Ethynyl-2’-deoxyuridine; oe-NC, overexpressed-negative control.