Figure 9.

Formation of MDA and carbonyl groups of baicalein in the presence of Cu (II).

Notes: (A) Reaction mixture (2.0 mL) contained 50 mM phosphate buffer (pH 7.4), 1mM 2-Deoxyribose, 100 μM Cu (II) and indicated concentration of baicalein. The reaction mixture was incubated at 37°C for 120 min. MDA formation was measured by determining the TBA reactive material. (B) Reaction mixture (2.0 mL) contained 50 mM phosphate buffer (pH 7.4), 2 mg·mL⁻¹ BSA, 100 μM Cu (II) and indicated concentration of baicalein. The reaction mixture was incubated at 37 °C for 30 min. Carbonyl groups formation was measured by determining the DNPH reactive material. All data were expressed as the means ± SD of values from triplicate experiments. **P<0.01 and ***P<0.001versus untreated group; ^#P<0.05, ^##P<0.01 and ^###P<0.001 for the significance between the groups with or without Neo and TU pretreatment.